TB-500 Reconstitution & Dosage Calculator

This calculator does one job. It turns your vial size, the amount of BAC water you add, and your target dose into three numbers: concentration in mg/mL, the volume you draw in mL, and the matching units on a U-100 insulin syringe.

TB-500 ships as a freeze-dried powder. Before it can be drawn into a syringe, it has to be mixed with bacteriostatic (BAC) water. The amount of water you add sets the concentration, and the concentration decides how many units you draw.

TB-500 doses are larger than most healing peptides, often around 2 to 2.5 mg. At weak mixes a single dose can fill a whole 1 mL syringe, so the calculator warns you when a draw is too big and tells you to mix stronger.

Start with a TB-500 preset when it matches your vial. Use custom fields only when your vial size, BAC water, or target dose is different from the preset options.

The math is short. Concentration is vial size divided by BAC water. Draw volume is target dose divided by concentration. U-100 units are milliliters multiplied by 100.

Here is one example. A 10 mg vial with 2 mL of BAC water gives 5 mg/mL. A 2.5 mg dose is 2.5 divided by 5, which is 0.5 mL, or 50 units. The same vial with 1 mL of water is 10 mg/mL, so 2.5 mg becomes 0.25 mL, or 25 units - a smaller, tidier draw.

Community research planning often describes TB-500 in two phases: a loading phase of about 2 to 2.5 mg twice weekly for several weeks, then a maintenance phase of about 2 to 2.5 mg once weekly. We list these numbers because people search for them. This is not a dose recommendation, and the calculator does not tell you which dose to use.

Below is how common mg doses convert to U-100 syringe units at two research concentrations. Remember 1 mg equals 1000 mcg.

TB-500 is a short synthetic fragment built around the actin-binding part of thymosin beta-4. Most human research used the full 43-amino-acid thymosin beta-4 protein, not the fragment. So the human safety and healing data you read about often come from the full peptide, not from TB-500 itself.

Research planning usually splits TB-500 into two phases. A loading phase uses a higher amount, often around 2 to 2.5 mg twice weekly, to build levels up. A maintenance phase then drops to about once weekly. The calculator does the unit math for whatever dose you enter; it does not pick the phase for you.

Add the BAC water slowly down the side of the vial and swirl gently. Do not shake, because foam makes an accurate draw impossible. Mixed TB-500 should look clear and colorless. Store it in the refrigerator and use it within about 4 weeks. Do not freeze a mixed vial.

TB-500 and BPC-157 are often discussed together for recovery research. If you use both, reconstitute and draw them separately unless you bought a pre-blended combo vial. To plan BPC-157 on its own, use the BPC-157 calculator. Note that TB-500 is banned in sport under WADA, so it can cause a failed drug test.