MOTS-c Reconstitution & Dosage Calculator

This calculator does one job. It turns your vial size, the amount of BAC water you add, and your target dose into three numbers: concentration in mg/mL, the volume you draw in mL, and the matching units on a U-100 insulin syringe.

MOTS-c ships as a freeze-dried powder. Before it can be drawn into a syringe, it has to be mixed with bacteriostatic (BAC) water. The amount of water you add sets the concentration, and the concentration decides how many units you draw.

MOTS-c is dosed two ways. Some protocols use small daily amounts in mcg, and others use larger weekly amounts in mg. Because of that range, you can enter your dose in mg or mcg, and the calculator warns you if a draw is too big for one syringe.

Start with the MOTS-c presets. They load common vial sizes, BAC water amounts, and dose entries. Use the custom fields only when your vial, water volume, or target dose does not match the preset you need.

The math is short. Concentration is vial size divided by BAC water. Draw volume is target dose divided by concentration. U-100 units are milliliters multiplied by 100.

Here is one example. A 10 mg vial with 2 mL of BAC water gives 5 mg/mL. A 1 mg dose is 1 divided by 5, which is 0.2 mL, or 20 units. But a 5 mg dose at that same 5 mg/mL is 1.0 mL, which fills a whole 1 mL syringe. For doses that big, add only 1 mL of water for a stronger 10 mg/mL mix.

MOTS-c shows up in research planning two ways. Some sources use small daily amounts, often 0.2 to 1 mg. Others use larger weekly amounts, often 5 to 10 mg, sometimes split or timed before exercise. We list these numbers because people search for them. This is not a dose recommendation, and the calculator does not tell you which dose to use.

Below is how common doses convert to U-100 syringe units at two research concentrations. Remember 1 mg equals 1000 mcg.

MOTS-c is a small peptide your mitochondria make. Research describes it as an exercise-like signal that switches on a cell pathway called AMPK, which is the same pathway exercise and the drug metformin use. That is why it is studied for metabolism, body composition, and aging. These are research findings, not proven results in people.

MOTS-c is written in mg for weekly doses and sometimes mcg for small daily doses. 1 mg equals 1000 mcg, so 0.5 mg is 500 mcg. Always check the unit before you draw, and the calculator accepts either.

MOTS-c often causes a red, raised welt at the injection site. To reduce that, some sources mix it with 0.9% saline (sodium chloride) bacteriostatic water instead of plain BAC water, and inject slowly. Rotating sites also helps. This is a handling note, not medical advice.

Add the water slowly down the side of the vial and swirl gently. Do not shake. Mixed MOTS-c should look clear. MOTS-c is more sensitive than some peptides, so many sources say store it in the refrigerator and use it within about 2 weeks. Do not freeze a mixed vial. A 10 mg vial gives two 5 mg doses or ten 1 mg doses.