Vial size
500 mg
Fluid volume
5 mL
Concentration
100 mg/mL
Units per 50 mg
50 units
Peptide Tools
Reconstitution + Units
NAD+ Reconstitution & Dosage Calculator
This free calculator turns NAD+ vial size, BAC water, and target dose into concentration, draw volume, and U-100 syringe units. It supports mg and mcg, and it is an educational research-planning tool, not medical advice. It does not tell you what dose to use.
Quick summary
- Converts NAD+ vial size (mg), BAC water (mL), and target dose (mg or mcg) into concentration, draw volume, and U-100 syringe units.
- Built for common NAD+ research vials, including 500, 1000, and 1500 mg setups.
- Educational research-planning tool only. It does not diagnose, treat, or recommend a dose.
- Compound
- NAD+ (nicotinamide adenine dinucleotide)
- Tool type
- Reconstitution and unit calculator
- Common research vials
- 500, 1000, 1500 mg
- Most common mix
- 500 mg + 5 mL = 100 mg/mL
- Regulatory status
- Research-use product, not FDA-approved as a therapeutic
NAD+ reconstitution calculator
NAD+ presets
Use the presets first; the manual steps below are for different vial sizes, water volumes, or doses.
3 mL max presets
500 mg + 5 mL = 100 mg/mL, so 25 mg = 25 units and 50 mg = 50 units and 100 mg = 100 units.
Mixed NAD+ should look clear. Buffered NAD+ is usually closer to neutral pH; unbuffered NAD+ can be acidic and may sting more.
Optional: use when your setup does not match a preset
Step 1
Syringe
U-100
Choose syringe size.
Step 2
Vial quantity
How many mg of peptides in your vial?
mg
Step 3
Dose
Dose amount per injection. 1 mg = 1000 mcg.
Step 4
BAC water
Bacteriostatic water used to reconstitute your vial.
mL
Your draw
1.0 mL
50units
0102030405060708090100
To have a dose of 50 mg, pull to 50 units.
Vial
500 mg
Water
5 mL
Volume
0.500 mL
Save this draw so you do not need to redo the math next time.
NAD+ shopping
Copy the discount code, then use at checkout.
Verified USA Suppliers
Verified EU Supplier
What this NAD+ calculator does
This calculator does one job. It turns your NAD+ vial size, the amount of BAC water you add, and your target dose into three numbers: concentration in mg/mL, the volume you draw in mL, and the matching units on a U-100 insulin syringe.
NAD+ usually ships as a freeze-dried powder or kit. Before a dry vial can be drawn into a syringe, it has to be mixed with the right sterile diluent. The amount of fluid you add sets the concentration, and the concentration decides how many units you draw.
NAD+ is usually planned in milligrams. The calculator also accepts mcg for people copying values from notes or labels. Always check the unit before you draw, because 1 mg equals 1000 mcg.
Research-use only - not medical advice
This page and calculator are educational research-planning tools. They do not recommend a dose, diagnose, or treat anything. NAD+ is not FDA-approved as a therapeutic. Talk to a qualified clinician before using any research compound.
How to use the NAD+ calculator
Start with the NAD+ presets. They load common vial sizes, water amounts, and dose entries. Use the custom fields only when your vial, water volume, or target dose does not match the preset you need.
- 1
Start with a NAD+ vial preset
Pick the NAD+ vial setup that matches your research vial and fluid amount. Common NAD+ research vials include 500, 1000, and 1500 mg.
- 2
Pick the NAD+ dose
Choose a common NAD+ dose entry, or type your own amount. The calculator fills the dose field without choosing or recommending a dose.
- 3
Use custom only if needed
If your setup does not match a preset, enter the vial size, water amount, or target dose by hand. Bigger NAD+ doses may need a stronger mix or a split draw.
- 4
Read the NAD+ draw
The calculator shows concentration in mg/mL, draw volume in mL, and matching U-100 syringe units. If the draw is larger than one syringe, the calculator warns you.
- 5
Save the NAD+ calculation
Use Save calculation to email yourself the vial, water, dose, unit result, and supplier links so you can check the math later.
NAD+ reconstitution math, explained
The math is short. Concentration is vial size divided by fluid volume. Draw volume is target dose divided by concentration. U-100 units are milliliters multiplied by 100.
Here is one example. A 500 mg vial with 5 mL of BAC water gives 100 mg/mL. A 50 mg dose is 50 divided by 100, which is 0.5 mL, or 50 units. The same vial with 10 mL of water is 50 mg/mL, so 50 mg becomes 1.0 mL, or 100 units.
Concentration and units per 50 mg by vial size and fluid volume
Vial size
500 mg
Fluid volume
10 mL
Concentration
50 mg/mL
Units per 50 mg
100 units
Vial size
1000 mg
Fluid volume
10 mL
Concentration
100 mg/mL
Units per 50 mg
50 units
Vial size
1500 mg
Fluid volume
15 mL
Concentration
100 mg/mL
Units per 50 mg
50 units
Vial size
1500 mg
Fluid volume
30 mL
Concentration
50 mg/mL
Units per 50 mg
100 units
A 100 mg/mL mix keeps a 50 mg draw at 0.5 mL. A 50 mg/mL mix makes the same 50 mg draw a full 1 mL syringe.
NAD+ dose-to-units reference
NAD+ research planning commonly uses milligram-size entries. We list these numbers because people search for them. This is not a dose recommendation, and the calculator does not tell you which dose to use.
Below is how common dose amounts convert to U-100 syringe units at two simple concentrations. Always check your own vial size and fluid amount in the calculator.
Units to draw at 100 mg/mL
Dose
25 mg
Volume (mL)
0.25 mL
U-100 units
25 units
Dose
50 mg
Volume (mL)
0.50 mL
U-100 units
50 units
Dose
100 mg
Volume (mL)
1.00 mL
U-100 units
100 units (full 1 mL syringe)
Dose
200 mg
Volume (mL)
2.00 mL
U-100 units
Will not fit one 1 mL syringe
At 100 mg/mL, 25 and 50 mg are easy to read on a U-100 syringe. Larger doses may need a split draw.
Units to draw at 50 mg/mL
Dose
25 mg
Volume (mL)
0.50 mL
U-100 units
50 units
Dose
50 mg
Volume (mL)
1.00 mL
U-100 units
100 units (full 1 mL syringe)
Dose
100 mg
Volume (mL)
2.00 mL
U-100 units
Will not fit one 1 mL syringe
Dose
200 mg
Volume (mL)
4.00 mL
U-100 units
Will not fit one 1 mL syringe
A 50 mg/mL mix is easier to dilute but quickly creates large draws.
Buffered vs unbuffered, comfort, and storage
Buffered and unbuffered NAD+ use the same math
The calculator math does not change when the vial is buffered. Buffered and unbuffered NAD+ use the same concentration formula. The buffer changes pH and handling, not the mg-to-units calculation.
Why NAD+ can sting
Unbuffered NAD+ can be acidic after reconstitution, often around pH 3 to 4. That is why many people report stinging or burning with NAD+ injections. Buffered NAD+ is usually closer to neutral pH and may be more comfortable.
Mixing and storage
Add the fluid slowly down the side of the vial and swirl gently. Do not shake. Mixed NAD+ should look clear. Store it cold, protect it from light, and follow the supplier or pharmacy beyond-use date.
NAD+ supplies
Use this as a simple shopping checklist for reconstitution. Confirm buffered status, vial size, batch documents, and current pricing before ordering. This is not dose advice.
Recommended Supply
PEPPAL applies to eligible supplier checkout links when supported by the supplier.
Why choose Peptide Partners?Verified Supplier LinkVerified Supplier Link
NAD+ Supply
View SupplierMore Supplies
Disclosure: supply links may earn PepPal a commission at no cost to you.
Frequently Asked Questions
What does this NAD+ calculator tell me?
It converts vial size, fluid volume, and target dose into concentration (mg/mL), draw volume (mL), and U-100 syringe units. It accepts mg or mcg. It does not tell you what dose to use.
Is this NAD+ reconstitution calculator free?
Yes. It runs in your browser at no cost and does not require an account.
How much BAC water do I add to a 500 mg NAD+ vial?
It depends on the concentration you want. A 500 mg vial with 5 mL makes 100 mg/mL, where 50 mg is 50 units. Using 10 mL makes 50 mg/mL, where 50 mg is a full 100-unit syringe. Enter your numbers and the calculator shows the result.
How many units is 50 mg of NAD+?
At 100 mg/mL it is 50 units. At 50 mg/mL it is 100 units. Units always depend on your concentration, so check your own mix in the calculator.
Does buffered NAD+ change the calculator math?
No. Buffered and unbuffered NAD+ use the same mg-to-mL formula. The buffer changes solution pH and handling, not the concentration math.
Why does NAD+ sting after reconstitution?
Unbuffered NAD+ can be acidic after reconstitution, often around pH 3 to 4. Buffered NAD+ is usually closer to neutral pH and may be more comfortable. Injection rate and concentration also affect comfort.
Why is my NAD+ draw bigger than my syringe?
NAD+ doses can be large, and a weak mix makes a big draw. At 50 mg/mL, a 100 mg dose is 2 mL and will not fit one 1 mL syringe. Use the calculator to compare stronger mixes or split draws.
Is NAD+ FDA-approved?
Injectable NAD+ is not FDA-approved as a therapeutic. Research-use NAD+ products are not approved drugs.
Is this medical advice?
No. This calculator is for education and research planning only. It does not diagnose, treat, or recommend a dose. Talk to a qualified clinician before using any research compound.
Preferred supplier
Peptide PartnersNeed peptides? Start with a verified supplier.
PepPal's recommended source with current discount access and established testing standards.
3rd-party testedResearch-grade supplierHigh purity batches
Sources and research notes
- 1. Schaefer Z, et al. Long-Term Stability of Nicotinamide Cofactors in Common Aqueous Buffers: Implications for Cell-Free Biocatalysis. ACS Synthetic Biology / NREL technical report (PMC11597533) (2024)
- 2. U.S. National Library of Medicine / DailyMed Bacteriostatic Water for Injection USP - pH 5.7 (range 4.5-7.0), 0.9% benzyl alcohol preservative. DailyMed (USP monograph) (2026)
- 3. Cleveland Clinic Health Library NAD (Nicotinamide Adenine Dinucleotide): function, anatomy, and clinical context. Cleveland Clinic (2026)
- 4. Yoshino J, Baur JA, Imai SI. NAD+ Intermediates: The Biology and Therapeutic Potential of NMN and NR. Cell Metabolism (2018)
- 5. Covarrubias AJ, Perrone R, Grozio A, Verdin E. NAD+ metabolism and its roles in cellular processes during ageing. Nature Reviews Molecular Cell Biology (2021)